Figure 2: NCLX expression and function in ameloblasts.
A) mRNA expression of Slc8b1 (coding for NCLX) in secretory and maturation stage ameloblasts (n = 6 animals). B) Original traces showing mitochondrial Ca2+ accumulation in secretory and maturation stage ameloblasts loaded with the mitochondrial Ca2+ indicator rhod2AM (4 μM) in the presence/absence of the NCLX inhibitor CGP-37157 (10 μM). C) Quantification of the Ca2+ basal level. Data represent the mean ± SEM of 3 independent experiments, 4 wells for each condition with 20-50 cells per field. Data were analyzed by 2 - tailed unpaired Student’s t test and one-way ANOVA. **P < 0.01, ***P < 0.001, n.s., non-significant.