| Acrylamide concentration | 20% | 10% | 8% | 6% | 5% | 4% |
|---|---|---|---|---|---|---|
| 20% denaturing gel solution (see ‘Reagent setup’) | 20 mL | 10 mL | 8 mL | 6 mL | 5 mL | 4 mL |
| 0% denaturing gel solution (see ‘Reagent setup’) | 0 mL | 10 mL | 12 mL | 14 mL | 15 mL | 16 mL |
| Total gel volume | 20 mL | 20 mL | 20 mL | 20 mL | 20 mL | 20 mL |
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