| Component | Amount (μL) | Final concentration |
|---|---|---|
| 25 purified DNA strands (10 μM, from Step 42) | 2 (of each strand; 50 total) | 100 nM |
| 10× TAE-Mg2+ buffer (Buffer 4) | 10 | 1× |
| Nuclease-free water | 40 | |
| Total | 100 |
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