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. 2022 Feb 17;42(2):e00459-21. doi: 10.1128/mcb.00459-21

FIG 4.

FIG 4

Highly heterogenous sensitivities of client proteins in different normal cells in response to ATP-binding inhibitor. Four normal cell lines were treated without (vehicle alone) or with 100 nM 17-DMAG for 48 h. Total cell lysates were equalized (within each cell line itself) for total cellular proteins and subjected to Western immunoblotting analysis with the indicated antibodies. Measurements of 17-DMAG-caused changes in client protein levels were restricted within each of the cell lines without or with 17-DMAG treatment. The results were reproducible in multiple independent repetitions of the same experiment.