FIG 8.

Hsp90 levels determine cell sensitivity or resistance to ATP-binding inhibitor. (A) The lowest Hsp90-expressing NDF cells were exogenously overexpressed with GFP-Hsp90α, and (B) the highest Hsp90-expressing 293T cells had an shRNA introduced to downregulate the endogenous Hsp90α. Both cell lines were then subjected to a cell survival assay with increasing dosages of 17-DMAG and compared to their wild-type counterparts. The results shown represent one of three repeated experiments with data in excellent agreement. Data are means and SD. P < 0.05, as significant.