Table 1.

Practical and theoretical numbers of vials/bags cryopreserved for selected cMSCs

cMSCs	(No. of cells × 106)/ml of freezing medium in each cell bank*
	ICB (0.5 ± 0.1)/0.5	MCB** (5 ± 0.5)/2	WCB** (10 ± 1)/4	EoPCB*** (75 ± 5)/15	EoPCB**** Estimated
D2C4	30	65	70	9	436 800
D5C1	36	80	80	10	760 320
D5C3	36	80	75	9	691 200

^*ICB: Initial cell bank, MCB: Master cell bank, WCB: Working cell bank, EoPCB: End of product cell bank, cMSCs: Clonal mesenchymal stromal cells

^**Number in the column is one vial of the previous tier

^***Number in the column is three vials of the previous tier

^****The gray column shows the estimated number of the final products based on experimental data from each batch