Table 4.

Validation of transport with dry ice inside a Styrofoam box*

Evaluated parameter	Method	Acceptance criteria	Results
			Replicate 1	Replicate 2	Replicate 3
Temperature	Data logger	-70 to -80 °C	Maintaining temperature integrity (h)
			16	15	16
Leaking	Visual inspection	Negative	Negative	Negative	Negative
Packaging	Visual inspection	Intact	Intact	Intact	Intact
Viability	Hemocytometer	≥ 90%	96%	98%	96%
Sterility (aerobic, anaerobic, and fungi)	Culture	Negative	Negative	Negative	Negative

^*Box dimensions: 29 × 16 × 12 cm³ internal size and 33 × 20 × 17 cm³ external size