FIG 1.

ER‐retention of GCase in neurons overexpressing GBA1 and in fibroblasts from a patient with Gaucher disease. (A) Western blot analysis of lysates treated with endo H, PNGase F, or untreated from patient‐derived dopaminergic neurons after lentiviral‐mediated over expression of GBA1 for 2 weeks at MOI of 7.5 or 15 and control neurons treated with lentivirus‐expressing GFP. (B) Western blot analysis of lysates treated with Endo H, PNGase F, or untreated from fibroblasts for control or patients with Gaucher disease type I (N370S/N370S) or type II (L444/L444P). CTRL, control; Endo H, Endoglycosidase H; ER, endoplasmic reticulum; GCase, glucocerebrosidase; GFP, green fluorescent protein; iPSC, induced pluripotent stem cell; MOI, multiplicity of infection; wt, wild type.