Fig 3. Expression of arnB in response to meropenem treatment is dependent on PhoPQ.

(A) Relative expression reverse-transcription quantitative PCR (qRT-PCR) of PhoPQ regulon transcripts after meropenem exposure. Each experiment was independently replicated three times (individual data points of the three experiments are reported here). (B) Strains carrying transcriptional P_arnB^:msGFP fusions were exposed to indicated conditions and analyzed on a C6 Accuri flow cytometer. Statistical difference between populations was determined with a one-way ANOVA followed by Tukey’s correction for multiple comparisons (ns, not significant; ****, p ≤ 0.0001).