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. 2022 Feb 17;17(2):e0263285. doi: 10.1371/journal.pone.0263285

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© 2022 Hodrea et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — mRNA expression of (A) transforming growth factor β (Tgfb1), (B) platelet-derived growth factor (Pdgfb), and (C) connective tissue growth factor (Ccn2) of control, diabetic and dapagliflozin treated diabetic rats (D+DAPA). (D) mRNA expression of Serpine1 (also known as plasminogen activator inhibitor-1). (E) Protein level of α-smooth muscle actin (α-SMA). Proteins were normalized to total protein Ponceau S staining as loading control. (F) mRNA expression of fibronectin (Fn). mRNA expressions were normalized to Rn18S mRNA expression. Bars indicate means±SDs and data were analyzed by one-way ANOVA with Holm-Sidak multiple comparisons test (n = 6/group) *p<0.05 vs. Control, **p<0.01 vs. Control, ***p<0.001 vs. Control, ^§p<0.05 vs. Diabetic, ^§§p<0.01 vs. Diabetic, ^§§§p<0.001 vs. Diabetic.