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. 2022 Feb 17;90(2):e00568-21. doi: 10.1128/iai.00568-21

FIG 2.

FIG 2

Quantification of NTHi and P. aeruginosa (Pa) polymicrobial biofilms. (A and B) Bacterial density within fluorescent biofilms was quantified using BiofilmQ software to measure biofilm volume of P. aeruginosa in dual- and single-species biofilms (A) and the mean cube surface of P. aeruginosa (quantified as 1 cube equals 1 μm) (B). (C and D) Cubing can be visualized as local density of P. aeruginosa in both polymicrobial NTHi/P. aeruginosa biofilms (C) and P. aeruginosa biofilms (D). Values are mean ± SEM (n = 4). *, P < 0.05, Mann-Whitney test, two-tailed.