FIG 4.

NTHi impact on subsequent P. aeruginosa (Pa) infection is strain independent. BALBc/J mice were intratracheally infected with spectinomycin-resistant NTHi 86-028NP (NTHi 86r) followed by mPA (mPA 08-31). (A) Bacterial counts in the lung homogenates were enumerated by viable colony counting 72 h postinfection. Similarly, intratracheal infections were also performed with spectinomycin-resistant NTHi HI-1 (NTHi HI-1r) and nonmucoid P. aeruginosa (FRD1mucA+). (B) Bacterial counts in the lung homogenates were enumerated by viable colony counting 72 h postinfection. Values are mean ± SEM (n = 10). LOD, limit of detection. *, P < 0.05, Kruskal-Wallis test with Dunn’s multiple-comparison test.