FIG 6.

Inflammatory cytokine levels after dual infection are reduced, compared to P. aeruginosa (Pa) infection alone. Cytokine and chemokine analyses were performed on BALF supernatants from infected or mock-infected mice 72 h postinfection using DuoSet ELISA kits for MCP-1 (A), TNF-α (B), IL-10 (C), IL-1α (D), IL-1β (E), and IL-6 (F). Values are mean ± SEM (n = 7 to 10). Dual infection indicates sequential introduction. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, one-way ANOVA with Tukey’s multiple-comparison test for post hoc analysis.