FIG 2.

Intestinal epithelial HIF-1 loss does not affect systemic infection, weight loss, or inflammatory responses in a Salmonella infection model. (A and B) Quantification of systemic Salmonella spread (normalized CFU per gram of tissue) in the liver, spleen, mesenteric lymph nodes (MLN), small intestine (SI), cecum, and colon and weight loss (over the time course of infection) of WT littermates and Hif1a^IEC mice harboring a constitutive HIF-1α knockout in IECs at 4 days postinfection (n = 7) (A) and WT littermates and tamoxifen-inducible HIF-1α-deficient (Hif1a^IECind) mice (n = 6) (B). (C) mRNA expression of Cxcl2, Il18, Il1b, Cxcl5, and Nos2 relative to β-actin in WT and Hif1a^IEC IECs of control mice (n = 5) and 4 days after infection with Salmonella Typhimurium (S. Tm) (n = 7) normalized to the WT control. Data represent means with SEM. *, P < 0.05; **, P < 0.01 (according to a Kruskal-Wallis test with Dunn’s posttest [A to C]).