FIG 3.

Myeloid cell-specific HIF-1α deletion affects Cxcl2 expression in IECs but not systemic Salmonella infection. (A and B) Quantification of systemic Salmonella spread/organ CFU after 4 days of infection (A) and quantification of weight loss during the time course of infection of WT littermates and Hif1a^MC mice harboring an HIF-1α knockout in myeloid cells (n = 4). (C) mRNA expression of Cxcl2, Il18, Il1b, Cxcl5, and Nos2 relative to β-actin in IECs from WT and Hif1a^MC uninfected controls (n = 3) and 4 days after infection with Salmonella Typhimurium (S. Tm) (n = 4) normalized to the WT control. Data represent means with SEM. *, P < 0.05; **, P < 0.01 (according to a Kruskal-Wallis test with Dunn’s posttest or Mann-Whitney U test [A {cecum}]).