FIG 5.

E. coli but not Salmonella intracellular survival in macrophages is HIF-1 dependent. (A) Intracellular survival of Salmonella in bone marrow-derived macrophages (BMDMs) from WT littermates and HIF-1α-deficient (Hif1a^MC) mice over a time course of 4 h (MOI of 10) (n = 3). Extracellular bacteria were killed by the addition of medium containing gentamicin after 30 min of incubation. (B) Intracellular survival of E. coli in WT and Hif1a^MC BMDMs over the time course of infection (n = 4). (C) Cell death analysis measured by lactate dehydrogenase (LDH) release into the surrounding medium of the controls and Salmonella- and E. coli-infected BMDMs after 2 and 4 h (absorbance [A₄₉₀ − A₆₈₀]). Data represent means with SEM. *, P < 0.05; **, P < 0.01 (according to a Kruskal-Wallis test with Dunn’s posttest [A to C]).