FIG 1.

The intimin-like adhesin locus contains predicted host cell interaction domains and is highly conserved among UPEC strains. (A) Phyre2-predicted structures of various regions of SinH, SinI, and RatA putative proteins are represented. Only domains predicted with greater than 70% confidence are shown. Green-highlighted regions indicate homology to known cell adhesion domains, light blue indicates sugar-binding domains, dark blue indicates intimin/invasin domains, purple indicates plectin-related domains, yellow indicates lipase domains, and orange indicates integrin-binding domains. Exact parameters for predicted regions are given in Table 1. (B) A phylogenetic tree was created in PATRIC to display the homology of SinH to other bacterial strains. CFT073 SinH is more similar to S. enterica (SivH) than are other UPEC strains, UTI89, F11, and UPEC536. (C) PATRIC protein blast tool was used to compare the SinH-RatA CFT073 locus to other prototype UPEC strains. The colors indicate the percent amino acid homology in each strain, ranging from 95% to 100% identity.