TABLE 1.
Strains and plasmidsa
| Strain or plasmid | Characteristic(s) | Reference |
|---|---|---|
| Strains | ||
| C600 | K-12 derivative | 81 |
| MG1655 | K-12 derivative | 82 |
| 0.1229 | O18:H1, B2 phylogroup; Ampr Tetr | 15 |
| 0.1229ΔB17::cat | p0.1229_2ΔmcbABCDEFG::cat; Ampr Tetr Camr | 15 |
| 0.1229ΔB17::FRT | p0.1229_2ΔmcbABCDEFG::FRT; Ampr Tetr | This study |
| 0.1229ΔmctAB | p0.1229_3Δ2850–5473::cat; Ampr Tetr Camr | 15 |
| 0.1229ΔB17ΔmctAB | p0.1229_2ΔmcbABCDEFG::FRT p0.1229_3Δ2850–5473::cat; Ampr Tetr Camr | This study |
| 0.1229Δfur | Δfur::cat; Ampr Camr | This study |
| PA2 | O157:H7 stx2a | 77 |
| PA2ΔcirA | ΔcirA::kan; Kanr | This study |
| PA2.1 | cirAp.Gln24ArgfsTer35 | This study |
| PA2.2 | cirAc.-329_-244del | This study |
| PA2.3 | cirAp.Leu35GlyfsTer51 | This study |
| EDL933 | O157:H7 stx1a stx2a | 83 |
| EDL933ΔtonB | ΔtonB::cat; Camr | This study |
| Plasmids | ||
| pKD3 | Camr oriRγ | 67 |
| pKD4 | Kanr oriRγ | 67 |
| pKD46 | ParaC-λ recombinase; Ampr | 67 |
| pCP11B | FLP recombinase; Kanr | 84 |
| pKD46-Kan | Kanr | 15 |
| pBAD24 | ParaC; Ampr | 85 |
| pKP315 | pBAD24::ParaC-tonB; Ampr | 86 |
| pBR322 | Ampr Tetr | 87 |
| pBR322::cirA | Ampr Tetr | This study |
| pBR322::mcc1229 | pBR322::p0.1229_32745–7950; Tetr | 15 |
| pBR322::mcc1229ΔmctA | pBR322::p0.1229_32745–7950Δ(3084–3792); Tetr | This study |
| pBR322::mcc1229ΔmctB | pBR322::p0.1229_32745–7950Δ(3831–5423); Tetr | This study |
| pBR322::mcc1229ΔmctC | pBR322::p0.1229_32745–7950Δ(5426–6319); Tetr | This study |
| pBR322::mctAB | pBR322::p0.1229_32745–5425; Tetr | This study |
| pBR322::mctABC | pBR322::p0.1229_32745–6343; Tetr | This study |
| pBR322::mctC | pBR322::p0.1229_35426–6343; Tetr | This study |
| pBR322::mctCDE | pBR322::p0.1229_35426-7047; Tetr | This study |
| pBR322::mctE | pBR322::p0.1229_37047–6703; Tetr | This study |
| pBR322::mctD | pBR322::p0.1229_36706–6320; Tetr | This study |
| pBR322::mctDE | pBR322::p0.1229_37047–6320; Tetr | This study |
| pRS551 | Promoterless lacZ; Ampr Kanr | 69 |
| pRS551::mctA′-lacZ | Ampr Kanr | This study |
| pRS551::mctB′-lacZ | Ampr Kanr | This study |
| pRS551::mctC′-lacZ | Ampr Kanr | This study |
| pRS551::mctE′-lacZ | Ampr Kanr | This study |
| pACYC184 | Tetr Camr | 88 |
| pACYC184::PmctA-mctA | Camr | This study |
| pACYC184::PmctA-mctB | Camr | This study |
| pACYC184::PmctA-mctC | Camr | This study |
a
Amp, ampicillin; Tet, tetracycline; Cam, chloramphenicol; FRT, flippase recognition target; Kan, kanamycin.