FIG. 4.

Western blot analyses of Hobetsu-type B. microti-like parasites isolated from various places in Japan. The respective parasite strains analyzed were Ho226, Ho232, Ho233, Ho234, Ho235, Ho236, Ho237, and Ho240 from Hobetsu in Hokkaido Prefecture (lanes 1 to 8); Ya501 from Yamanaka in Shiga Prefecture (lane 9); An2 and An3 from Anan in Tokushima Prefecture (lanes 10 and 11); Da111, Da112, and Da116 from Daito in Shimane Prefecture (lanes 12 to 14); Ot1 and Ot2 from Ohtaki in Chiba Prefecture (lanes 15 and 16); and Aw3 and Aw6 from Awaji in Hyogo Prefecture (lanes 17 and 18). Parasite antigens were probed with a convalescent-phase serum from a hamster that was infected with strain Ho234.