Fig. 6.

Fluorescence images of mc3t3e1cell in the cell culture supernatants following exposure to (B) 0-d leachates, (C) 10-d leachates and (D) 15-d leachates with a concentration of 100 μL leachates/1000 μL cell culture. Cell culture supernatants with adding deionized water (100 μL deionized water/1000 μL cell culture) were used as a negative control (A). Mc3t3e1cells show intracellular green 2′,7′-dichlorofluorescein (DCF) fluorescence as a result of ROS production. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)