Fig. 3.

A IAA concentration of the culture supernatant of the three fungi grown with and without tryptophan. CF fungal culture filtrate. Using OD values of fungal culture filtrates, concentration of auxin was calculated through standard curve formula X = Y + 0.010/ 0.018 (X = conc. of IAA to be calculated, Y = mean ± SE of three replicates of OD values for each assay in triplicate, n = 9), B colour change in reaction mixture having CJAN1179 CF with tryptophan after incubation, Blank = reaction mixture without standard or test sample, Standard = 0.01% Indole-3-acetic acid solution, Test = reaction mixture having different volumes (5–30 µl) of sample (CJAN1179 CF with 0.1% L-tryptophan). Reaction mixture = Test/ standard/blank + H₂O + Salkowski reagent = 250 µl