Figure 2: Multiplexed imaging has uncovered novel spatial markers and cell-cell interactions underlying diseased states.

In this schematic, two representative tissue samples are compared. Healthy tissue is represented by a blue background and diseased tissue by a yellow background. Multiplexed imaging studies have revealed i) differential cell-type abundance in healthy and diseased tissue with distinct cell types present in diseased tissue that are not present or that are present at different levels in healthy tissue; ii) differences in local cell density iii) restriction of certain phenotypic markers to healthy or diseased cells; iv) spatially restricted expression; v) co-localization of certain cell types only in healthy or diseased tissue; and vi) unique distance relationships between cell types.