Scheme 1.
Structure and function of 3MBP5. a) Molecular structure of 3MBP5. b) 3MBP5 is used for the simultaneous visualization of SARS‐CoV‐2 Mpro and PLpro, and rapid inhibitor screening. i) Mpro and PLpro plasmid transfected or SARS‐CoV‐2‐infected cells can produce Mpro and PLpro; ii) 3MBP5 was incubated with the transfected or infected cells; iii) after being cleaved by Mpro and PLpro, the fluorescence of the Cy3 and Cy5 fragments recovered without spatial proximity to the BHQ‐2 quencher; iv) 3MBP5 fluorescence remains quenched when the cells were incubated with Mpro and PLpro inhibitors.