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. 2022 Feb 17;13:914. doi: 10.1038/s41467-022-28611-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative photomicrographs of oocytes from NA-POF mice treated with or without Ab4B19. Note that there are much more abnormal oocytes in a vehicle-treated group than the Ab4B19-treated group. Black arrow: abnormal oocytes. Scale bar, 100 μm. b Quantification of abnormal oocytes by the percentage of abnormal oocytes/total ovulated oocytes per mouse. N = 8 mice per group. c Representative images of the embryo implantation sites from NA-POF mice in vehicle-treated group, Ab4B19-treated group, and non-pregnant mice used as the control. These mice were sacrificed 7.5–8.5 days after vaginal plug detection. The red arrowheads indicated the implanted embryos in the uterus. d Quantification of the number of implanting embryos per mouse. N = 8 mice per group. e, f Quantitative analysis of fertility parameters. Female mice were treated with vehicle or Ab4B19 for a period of over 2 months until they got pregnant and their pups were born. Total numbers (e) and body weight (f) of newborn pups in vehicle and Ab4B19-treated groups (N = 10 female mice in e; N = 8 pups in vehicle group and n = 31 pups in Ab4B19-treated group). g Novel object recognition test for the first-generation offspring from Ab4B19-treated NA-POF group. Normal mice of the same age were used as the control. N = 5 mice per group. Data were presented as mean ± SEM. Statistical analyses were carried out by two-tailed student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001; ns not significant; b, d–g). Source data are provided as a Source Data file.