Fig. 5. Alleviation of ovarian defect and gonadotoxicity by Ab4B19 in Cy-POF model.

a Experimental design of Ab4B19 treatment and analysis in Cy-POF mice. b H&E staining ovaries (midline sections) harvested from a normal female mouse (left) or a Cy-POF mouse treated with vehicle (center) or Ab4B19 (right) for 6 days. Asterisks: the corpora lutea; Red arrow: atretic ovarian follicle. Scale bars, 200 μm. c Quantification of primordial, pre-antral, antral follicles, atretic follicles, and corpora lutea per ovary in normal mice (Ctrl), and Cy-POF mice treated with vehicle or Ab4B19. N = 8 ovaries per each condition. Unless stated otherwise, statistical analyses for comparison among three or more groups in this and all the following figures were carried out using one-way ANOVA, followed by Dunnett’s multiple comparisons tests. d Western blotting showing the expression of cleaved and total caspase-3. The apoptotic level was determined by the normalized ratio of the cleaved to total caspase-3. N = 5 mice per group. e Immunofluorescence images of ovaries stained with cleaved caspase-3 antibody (Green: cleaved caspase-3, Blue: DAPI). Images in the red frames in the center row are magnified and shown on the right. Scale bars, 200 μm. f Quantification of apoptosis in pre-antral and antral follicles. Apoptosis index was expressed as apoptotic/total follicles (N = 5 mice per group). Data were all presented as mean ± SEM. Statistical analyses were carried out using one-way ANOVA followed by the Dunnett’s multiple comparisons test (c, d, f) (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are provided as a Source Data file.