Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 17;13(2):159. doi: 10.1038/s41419-022-04606-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A The mRNA expression of Nanog, CD44 and CD133 in NC and SF CRC cells by PCR. B, C Nanog expression in NC and SF CRC cells was tested by Western blotting (B) and Immunofluorescence staining (C). Scale bar = 20 μm. D Silencing Nanog decreased the cell viability of SF CRC cells. CRC cells were transfected with Nanog siRNAs or negative control siRNAs for 48 h before serum deprivation. Cell viability was detected by CCK8 and marked as SF 0–96 h. E, F Silencing Nanog relieved the G0-G1 arrest (E) and increased the apoptosis rate (F) in SF CRC cells. CRC cells were transfected with Nanog siRNAs or negative control siRNAs for 48 h, and serum deprived for 12 h successively, then collected to analyze in the FCM. G, H Nanog knockdown decreased the expression of COUP-TF1, hDEC2, P21 and P27. Data are shown as the means ± s.e.m., n = 3; ns: no statistical significance, **P < 0.01, ***P < 0.001. Data represented at least 3 independent experiments.