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. 2022 Feb 17;13(2):159. doi: 10.1038/s41419-022-04606-1

Fig. 6. FAO increases nuclear ACLY expression to activate Nanog via P300/H3K27Ac.

Fig. 6

A–C The expression of ACLY was analyzed by PCR (A), Western blotting (B) and Immunofluorescence staining (C) in normal CRC cells, in SF CRC cells and in SF CRC cells treated with ETO. Scale bar = 20 μm. D The expression of ACLY in cytoplasm and nucleus was analyzed by Western blotting. E, F ACLY knockdown decreased the expression of P300 and Nanog. Serum deprived HT29 cells were transfected with ACLY siRNAs or negative control siRNAs. The expression of ACLY, P300 and Nanog was analyzed by PCR. F Western blotting analysis of P300, H3K27Ac and Nanog expression by ACLY inhibition in serum-deprived CRC cells. Data are shown as the means ± s.e.m., n = 3; *P < 0.05, **P < 0.01, ***P < 0.001. Data shown represented three independent experiments.