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. 2022 Feb 4;13:821542. doi: 10.3389/fimmu.2022.821542

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Copyright © 2022 Du, Zhu, Jiao, Nian, Zhang, Zhou, Zheng, Tong, Wei and Fu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inducing human CD49a⁺ NK cells from three different sources. (A) Schematic diagram of CB-iNK cells (left) and BM-iNK cells (right) acquired from HSCs. (B) Representative density plots of CD34⁺ cells sorted from cord blood or bone marrow mononuclear cells, and flow cytometric analysis of the percentage of CD3^-CD56⁺ NK cells in the system on days seven through thirty-five. (C) Statistical analysis of CD34⁺ HSCs purity after MACS sorting. (D, E) Flow cytometry analysis of frequency (D) and absolute number (E) for CD3^-CD56⁺ NK cells on day 35 of culture. (F) Schematic diagram of pNK-iNK cells acquired from purified pNK cells. (G) Representative density plots of CD3^-CD56⁺ pNK cells and pNK-iNK cells. (H) Statistical analysis of CD3^-CD56⁺ NK cells. (I, J) The percentage of CD3^-CD56⁺ NK cells that expressed CD49a in each group was tested by flow cytometry. Representative density plots (I) and statistical calculation of all samples (J). Data was analyzed by one-way ANOVA. Data represent means ± SD, n ≥ 6 in each group. ****p < 0.0001; ns, not significant.