Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 17;13:912. doi: 10.1038/s41467-022-28623-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a The cytokine IL-12 was fused to RELEASE and placed under the control of TVMVP. b TVMVP significantly increase IL-12 secretion. c The inwardly rectifying potassium channel Kir2.1 was fused to RELEASE. In addition, the genetically encoded voltage indicator ASAP3 was co-transfected. d Co-expression of Kir2.1-RELEASE with TEVP resulted in a significant increase in the amount of Kir2.1 expressed on the surface, which was quantified using surface staining for HA and flow cytometry. The surface display of functional Kir2.1 in response to TVMVP was shown to cause hyperpolarization of transfected cells. This was validated by measuring change in the fluorescence intensity of the genetic reporter e ASAP3, or the chemical dye, f DiSBAC2(3). Each dot represents an individual biological replicate. Mean values were calculated from four (b) or three replicates (d–f). Error bars represent ±SEM. The results are representative of at least two independent experiments. Significance was tested using an unpaired two-tailed Student’s t-test between the two indicated conditions for each experiment. **p < 0.01, ***p < 0.001, ****p < 0.0001.