Fig. 5. KEAP1 and PGAM5 participate in the cell death of melanocytes under oxidative stress.

The cell viability of PIG1 cells treated by H₂O₂ or pretreated with KEAP1 siRNA prior to H₂O₂ disposition is determined by CCK8 assay (A) and Resazurin-conversion assay (B). C The activity of released LDH in PIG1 cells treated by H₂O₂ or pretreated with KEAP1 siRNA prior to H₂O₂ disposition, determined by LDH release assay. D Annexin V-FITC/PI apoptosis assay of PIG1 cells pretreated with KEAP1 siRNA, followed by treatment with H₂O₂. The cell viability of PIG1 cells treated by H₂O₂ or pretreated with PGAM5 siRNA prior to H₂O₂ disposition is determined by CCK8 assay (E) and Resazurin-conversion assay (F). G The activity of released LDH in PIG1 cells treated by H₂O₂ or pretreated with PGAM5 siRNA prior to H₂O₂ disposition, determined by LDH release assay. H Annexin V-FITC/PI apoptosis assay of PIG1 cells pretreated with PGAM5 siRNA, followed by treatment with H₂O₂. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.