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. 2022 Feb 17;13:945. doi: 10.1038/s41467-022-28593-1

Fig. 1. Stroke affects leukocytes in a compartment-specific fashion.

Fig. 1

A Scheme of the experimental approach. Middle cerebral artery occlusion (MCAO) (or sham-operated, as controls) was induced in wild-type (WT) mice for 30 or 45 min and mice were sacrificed 24 h or 72 h later (post ischemia). Prior to stroke induction, fluorophore-labeled CD45 antibody (mAb) was injected intravenously (iv). After 5 min, mice were intracardially perfused and CD45+-leukocytes negative for the CD45iv antibody (CD45+CD45iv−) then defined as tissue-resident leukocytes were flow-sorted from central nervous system (CNS) parenchyma, pia, dura, choroid plexus (CP) and blood, and analyzed by flow cytometry and single-cell RNA-sequencing (scRNA-seq). Tissue sections were analyzed by immunohistochemistry. B Frequencies of Bc (B cells), DC (dendritic cells), Mono/Macro (Monocytes/Macrophages), Granulo (Granulocytes), NK (Natural killer cells), Tc (T cells) and Micro (Microglia) of tissue-resident leukocytes isolated out of the Brain, CP, Dura and Pia from sham-operated mice without stroke (ctrl), from mice 24 h post ischemia (24 h) and from mice 72 h post ischemia (72 h) analyzed by Flow Cytometry. n = 4 for ctrl, n = 12 for 24 h and 72 h. Data are presented as mean values ± SD. Statistical significance was tested using Kruskal–Wallis test with Dunn’s post test. Not significant = not shown, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Source data for B are provided as a Source Data file.