TABLE 3.
HBV DNA PCR reactivity in consecutive patient serum samples compared to serology
| Sample no. | Resulta
|
||||
|---|---|---|---|---|---|
| HBV viral load (pg/ml) | HBsAg | a-HBs (IU/ml) | QIAamp + PCRb | Extractor + PCRc | |
| Patient A | |||||
| 1 | 1,427 | NT | NT | Pos | Pos |
| 2 | 42 | Pos | NT | Pos | Pos |
| 3 | 3.5 | NT | NT | Pos | Pos |
| 4 | 0.9 | Pos | NT | Pos | Pos |
| 5 | Neg | Pos | NT | Pos | Pos |
| 6 | Neg | Pos | NT | Neg | Pos |
| Patient B | |||||
| 11 | NT | Pos | NT | Pos | Pos |
| 12 | NT | Pos | 402 | Neg | Pos |
| 13 | NT | Neg | >1,000 | Neg | Neg |
| 14 | NT | Neg | >1,000 | Neg | Neg |
Pos; positive; Neg, negative; NT, not tested.
Qiagen extraction using 200-μl sample input.
10 min at 80°C extractor protocol using 1-ml sample input.