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. 2022 Feb 17;12:2727. doi: 10.1038/s41598-022-06721-7

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proximal (top) and distal (bottom) micrographs of Chinook salmon otoliths comprised of calcite and aragonite from fish 1084 and 5244 and two all aragonite otolith from fish 5294. The polarized cross-section panel shows a prepared (30 μm thick) thin section of 5244-b Chinook salmon otolith under cross-polarized light microscopy showing areas of calcite (C) and vaterite (V). The calcite is readily identified by its uniaxial (−) optic sign and large grain-sizes relative to vaterite.