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. 2022 Feb 17;12:2702. doi: 10.1038/s41598-022-06269-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LIM Tracker screen structure. (a) LIM Tracker screen structure. The screen is divided into detection mode and tracking mode, and tracking is performed by switching between them. The entire screen is divided into four panels, with image display and ROI setting functions (top left), analysis data display functions (top right), image display adjustment functions (bottom left), and analysis processing operation functions (bottom right) integrated into each panel. (b) The ROI and each data display item (list, graph, scatter plot, montage image, cell lineage) are linked, and the display is updated in real time when there is a change in the ROI status. Each item in the data display responds to mouse clicks, and all data related to the selected item are instantly highlighted.