Table 2.
List of primary and secondary antibodies used in the Ras activity assay.
| Antibody | Species | Source | Catalogue no. | Dilution |
|---|---|---|---|---|
| KRas | Mouse | LS Bio | C175665 | 1:2000 |
| pMEK S217/221 | Rabbit | Cell signalling | 9154 | 1:1000 |
| pERK T202/Y204 | Mouse | Cell signalling | 9106 | 1:1000 |
| pAKT (S473) | Rabbit | Cell signalling | 4060 | 1:1000 |
| BIM | Rabbit | Cell signalling | 2933 | 1:1000 |
| Vinculin | Mouse | Sigma | V9131 | 1:10,000 |
| pFRA (S265) | Rabbit | Cell signalling | 3880 | 1:1000 |
| p-EGFR (Y1068) | Rabbit | Cell signalling | 3777 | 1:1000 |
| Cleaved PARP | Rabbit | Cell signalling | 9541 | 1:1000 |
| pRSK T359/S363 | Rabbit | Cell signalling | 9344 | 1:1000 |
| Phospho-S6 Ribosomal Protein (S235/236) | Rabbit | Cell signalling | 4858 | 1:2000 |
| Anti-rabbit IgG HRP-linked | Goat | Cell signalling | 7074 | 1:5000 |
| Anti-mouse IgG HRP-linked | Goat | Cell signalling | 7076 | 1:5000 |