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. 2022 Feb 17;12:2673. doi: 10.1038/s41598-022-06411-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human AD seeding time course in RCNs in a 96-well assay format. High-content imaging shows how neuritic, thread-like aggregates form over time that can be detected with the rodent-specific Tau antibody, T49 (a). The percentage of effect expressed as the ratio between T49 positive aggregates, and the count of live nuclei has been normalised on the amount of aggregates at DIV 14. Scale bar 100um (b).