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. 2022 Feb 4;13:774387. doi: 10.3389/fphar.2022.774387

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Copyright © 2022 Morsy, El-Sheikh, Abdel-Hafez, Kandeel and Abdel-Gaber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nuclear factor-κB (NF-κB) immunohistochemical staining of the kidney after methotrexate (MTX) and/or paeonol administration. NF-κB antibody–stained sections of the rat kidney (×400) from (A) control, (B) paeonol-treated, (C) MTX-treated, and (D) MTX/paeonol-treated rats. (E) Percent of positive cells compared to the control group, obtained from sections of each animal (n = 6 for each group), six fields/section, where results were considered significantly different when p < .05. ^a,b Significant difference compared to control or MTX groups, respectively.