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. 2022 Feb 5;12(3):e4314. doi: 10.21769/BioProtoc.4314

Figure 4. CPE assay for HCoV-NL63.

Figure 4.

A. Titration of HCoV-NL63 in the Vero E6 cell line. B. Results of HCoV-NL63 titration in Vero E6 cell line in CPE assay. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's t-test). The S/B ratios are labeled for each virus dilution. C. Determination of EC50 values for GC-376 and remdesivir against HCoV-NL63 in the CPE assay. D. EC50 curve fitting for remdesivir obtained in GraphPad Prism 8. E. EC50 curve fitting for GC-376 obtained in GraphPad Prism 8. F. Titration of the HCoV-NL63 in Huh-7 cell line; G. Titration of HCoV-NL63 in MRC-5 cell line. H. Titration of HCoV-NL63 in RD cell line. The images are representatives of three independent repeats.