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Purification of Phαβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation exchange HPLC of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 (m/z).
