Figure 8.

Protein and mRNA expression of both TGF-β and Smad7. (A) Protein expression of TGF-β and Smad7 in the intestinal epithelium was detected by western blotting. In mice with DSS-induced colitis treated with anti-TNF-α and 5-ASA, TGF-β and Smad7 expression were increased and decreased, respectively. (B) TGF-β and Smad7 mRNA expression in the intestinal epithelium were detected by reverse transcription-quantitative PCR. In mice with DSS-induced colitis treated with anti-TNF-α and 5-ASA, TGF-β and Smad7 mRNA expression were increased and decreased, respectively. *P<0.05 vs. DSS. DSS, dextran sulfate sodium; 5-ASA, 5-aminosalicyclic acid.