Figure 1.

Overexpression of PTX3 inhibits the proliferation of HTR-8/SV neo and JEG3 cells. Expression levels of PTX3 following transfection with PTX3 plasmid in HTR-8/SV neo cells were evaluated by using (A) RT-qPCR and (B) western blot assay. Expression of PTX3 following transfection with PTX3 plasmid in JEG3 cells was determined by using (C) RT-qPCR and (D) western blot assay. Proliferation of (E) HTR-8/SV neo and (F) JEG3 cells following PTX3 overexpression were examined with Cell Counting Kit-8 assay. *P<0.05, **P<0.01, ***P<0.001 vs. Control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. Ov-NC. PTX3, pentraxin 3; RT-qPCR, reverse transcription-quantitative PCR; Ov, overexpression vector; NC, negative control.