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. 2022 Feb 7;25(4):115. doi: 10.3892/mmr.2022.12631

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — IL-1Ra suppresses the inhibitory effects of PTX3 overexpression on the invasion of HTR-8/SV neo and JEG3 cells. The invasion of (A) HTR-8/SV neo and (B) JEG3 cells treated with Ov-PTX3 and IL-1Ra was assessed by using Transwell invasion assay. The mRNA expression of MMP2 and MMP9 in (C) HTR-8/SV neo and (D) JEG3 cells following treatment with Ov-PTX3 and IL-1Ra was detected via reverse transcription-quantitative PCR. The protein expression of MMP2 and MMP9 in (E) HTR-8/SV neo and (F) JEG3 cells following treatment with Ov-PTX3 and IL-1Ra was determined via western blotting. ***P<0.001 vs. Control; ^##P<0.01, ^###P<0.001 vs. Ov-PTX3. IL-1Ra, interleukin-1 receptor antagonist; PTX3, pentraxin 3; Ov, overexpression vector.