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. 2021 Dec 21;50(1):72–91. doi: 10.1093/nar/gkab1137

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — MNase treatment assay of the NCPs containing the H3.3 mutants. (A) The NCPs containing H3.3 (lanes 2–7), H3.3R40C_R53S (lanes 8–13) and H3.3R72L_R83C (lanes 14–19) were incubated in the presence of MNase for 0, 3, 6, 9, 12 and 15 min. (B) The experiments with the NCPs containing H3.3 (lanes 2–7), H3.3R40C (lanes 8–13) and H3.3R53S (lanes 14–19). (C) The experiments with the NCPs containing H3.3 (lanes 2–7), H3.3I124T (lanes 8–13) and H3.3C-term (lanes 14–19). The resulting DNA fragments were deproteinized and analyzed by native-PAGE with ethidium bromide staining. The results were confirmed by three additional independent experiments. The arrow in panel (A) indicates the band (90-95 base pairs) corresponding to the DNA fragment produced by MNase cleavage around SHL2.5.