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. 2021 Dec 21;50(1):72–91. doi: 10.1093/nar/gkab1137

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Ectopic expression of H3mm18 suppresses myogenic differentiation in C2C12 cells. (A) Schematic illustration of the ectopic expression experiment. (B) ICC analysis of differentiated C2C12 cells. C2C12 cells expressing EGFP, EGFP-tagged H3.3 or H3mm18 (green in bottom images, N-terminally tagged) were differentiated for 72 h and stained with antibodies for the indicated proteins. To evaluate the fusion index, the total nuclei and those in MyHC-positive myotubes were counted for each field of view (FOV, >300 nuclei for each FOV, three FOVs for each cell type). Fusion index is graphed as % MyHC-containing nuclei. ***P < 0.001; one-way analysis of variance (ANOVA). (C) Immunoblot analysis of undifferentiated (Undiff) and differentiated (Diff) C2C12 cells expressing EGFP or EGFP-H3mm18.