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. 2021 Dec 21;50(1):72–91. doi: 10.1093/nar/gkab1137

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Ectopic expression of H3mm18 alters the transcription status in C2C12 cells. (A) Transcriptome analysis using CEL-Seq2. MA plots showing the effects of differential expressions of EGFP-H3mm18 on proliferative and differentiated states. Differentially expressed genes (DEGs) are depicted in red. (B) Gene ontology (GO) analysis on DEGs in differentiated cells. Top 10 GO terms for upregulated DEGs (left) and down-regulated DEGs (right panel) are listed. (C) A heatmap showing expression patterns of up- or down-regulated DEGs upon H3mm18 expression during differentiation. Actb, Gapdh, and Rpl27 are shown as non-DEG, housekeeping genes.