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. 2021 Dec 21;50(1):72–91. doi: 10.1093/nar/gkab1137

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — H3mm18 interacts with H3.3 chaperones. (A) Coimmunoprecipitation with FLAG-tagged DAXX. NIH3T3 cells expressing the N-terminally EGFP-tagged histone H3.1, H3.3 or H3mm18 were infected with retroviral vectors for C-terminally FLAG-tagged DAXX, and then treated with 5 μM MG132 for 6 h. Chromatin-unbound fractions were prepared and subjected to the immunoprecipitation (IP) assay using the anti-FLAG antibody. Input fractions and precipitates were analyzed by western blotting using the indicated antibodies. (B) IP for interaction with endogenous DAXX. NIH3T3 cells expressing the N-terminally EGFP-tagged histone H3.1, H3.3 or H3mm18 were treated with 5 μM MG132 for 6 h and subjected to IP, using anti-GFP or anti-H3mm18 antibodies.