(A) XZ-confocal image of a cross section of airway epithelial cells stained with calcein, a fluorescent green dye, and ASL stained with Texas Red Dextran, a cell impermeable red dye. (B) After administration of an osmotic stimulus (HS), there is an increase in ASL height along with a decline in cell height. (C) HS aerosolized to the surface of HBE cultures results in a significant but transient increase in ASL height, lasting under an hour. N=8. Rectangle represents duration of HS administration (8μg NaCl/cm2/min for 15 min).