Table 2. Kinetic Parameters Derived from ThT Experiments Shown in Figure 4a.
sample | Imax (A.U.) | tlag (min) | t1/2 (min) | k (min–1) | τ (min) |
---|---|---|---|---|---|
Aβ1–40 20 μM | 12.7 ± 0.2 | 239.0 ± 2.5 | 439.0 ± 2.8 | 2.5·10–2 ± 1.2·10–3 | 40.0 ± 2.3 |
Aβ1–40 20 μM/Cu2+ 1:1 | 21.5 ± 0.1 | 814,5 ± 3.2 | 986.9 ± 3.5 | 2.8·10–3 ± 1.0·10–4 | 357.1 ± 5.2 |
Aβ1–40 20 μM/Semax 1:1 | 10.5 ± 0.5 | 353.3 ± 5.6 | 462.0 ± 6.6 | 4.6·10–2 ± 1.8·10–3 | 21.7 ± 3.6 |
Aβ1–40 20 μM/Semax 1:5 | 10.5 ± 0.5 | 806.6 ± 4.3 | 865.4 ± 4.1 | 8.5·10–2 ± 2.2·10–3 | 11.8 ± 5.1 |
Aβ1–40 20 μM/Cu2+/Semax 1:1:1 | 3.7 ± 0.2 | 554.7 ± 2.1 | 706.2 ± 1.8 | 3.3·10–3 ± 1.2·10–4 | 303.0 ± 6.2 |
Aβ1–40 20 μM/Cu2+/Semax 1:1:5 | 5.8 ± 0.1 | 1407.7 ± 10.6 | 1635.0 ± 12.4 | 2.2·10–3 ± 2.3·10–4 | 454.5 ± 13.5 |
ThT curves were fitted by using the equation . Lag time was calculated as tlag = t1/2 – 2τ, and kapp was given by 1/τ. Results are the average of three independent experiments.