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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Immunol. 2022 Jan 14;208(3):745–752. doi: 10.4049/jimmunol.1901171

FIGURE 4.

FIGURE 4.

Monocyte recruitment in mixed bone marrow chimeras. (A) Schematic showing the establishment of CFTRΔF508/WT mixed bone marrow chimeras. (B-C) CFTRΔF508 and WT monocytes (CD115+CD11b+CD11c−F4/80−, B) and macrophages (F4/80+CD11b+, C) in the lungs of unchallenged mixed chimeric mice as assessed by flow cytometry. (D-E) Mixed chimeric mice were challenged with CCL2 (intranasal 100 ng/mice, 12 hours before the harvest) and the BAL/lung monocyte ratio (D) or Ly6Chi BAL/lung monocyte ratio (E) was calculated for CFTRΔF508 and WT monocytes. (F-G) The small intestinal lamina propria leukocytes (LPL) of mixed chimeric mice were harvested assessed for monocytes (CD11b+CD11c−F4/80−Ly6G−TCR−Cd19−NK1.1−, F) and macrophages (F4/80+, G) by flow cytometry. Mean±SD of n=10 mice for B-C, F-G and 14 mice for D-E. n.s. p>0.05, *p<0.05, **p<0.01 in the student T-test.