Table 5.
The information of primers used in this study
| Gene | Sequence (5,-3,) | Size (bp) | Description | Reference/Accession |
|---|---|---|---|---|
| cnf |
AAGATGGAGTTTCCTATGCAGGAG TGGAGTTTCCTATGCAGGAG |
498 | Cytotoxic necrotizing factor | Johnson & Stell. (2000) |
| feoB |
AATTGGCGTGCATGAAGATAACTG AGCTGGCGACCTGATAGAACAATG |
470 | Ferrous iron transporter | Yamamoto et al. (1995) |
| irp2 |
AAGGATTCGCTGTTACCGGAC AACTCCTGATACAGGTGGC |
413 | Yersiniatbactin biosynthesis | Ewers et al. (2005) |
| iroD |
AAGTCAAAGCAGGGGTTGCCCG GACGCCGACATTAAGACGCAG |
665 | Catecholate siderophore receptor | Johnson. (2000) |
| ibeA |
AGGCAGGTGTGCGCCGCGTAC TGGTGCTCCGGCCAACCATGC |
170 | Invasion of brain endothelium | Johnson & Stell. (2000) |
| fimH |
TGCAGAACGGATAAGCCGTGG GCAGTCACCTGCCCTCCGGTA |
508 | Type 1 fimbriae | Johnson & Stell. (2000) |
| LT |
TATCCTCTCTATATGCACAG CTGTAGTGGAAGCTGTTATA |
480 | Enterotoxins | Osman et al. (2012) |
| kpsM |
CATCATCAAATGGCAAGA AAGCAGTATCGGCAGGAC |
394 | capsular polysaccharide synthesis | AF007777.1 |
| ompT |
TCATCCCGGAAGCCTCCCTCACTACTAT TAGCGTTTGCTGCACTGGCTTCTGATAC |
496 | Outer member protein | MG149556.1 |
| iutA |
GGCTGGACATCATGGGAACTGG CGTCGGGAACGGGTAGAATCG |
302 | Iron acquisition system | JX466848.1 |
| iss |
CAGCAACCCGAACCACTTGATG AGCATTGCCAGAGCGGCAGAA |
323 | serum resistance | AF042279.1 |
| hlyA |
AACAAGGATAAGCACTGTTCTGGCT ACCATATAAGCGGTCATTCCCGTCA |
1177 | α-Hemolysin | Yamamoto et al. (1995) |
| ompA |
AAATACGGTAGAGTCAGGTGG CGTTCACGCTTAATAAATGG |
330 | Outer membrane protein | FJ158545.1 |
| TEM |
CATTTCCGTGTCGCCCTTATTC CGTTCATCCATAGTTGCCTGAC |
800 | β-lactamases resistance | Yao F et al.(2007) |
| SHV | AGCCGCTTGAGCAAATTAAAC ATCCCGCAGATAAATCACCAC | 713 | ||
| OXA | GGCACCAGATTCAACTTTCAAG GACCCCAAGTTTCCTGTAAGTG | 564 | ||
| CTX-M | CGCTTTGCGATGTGCAG ACCGCGATATCGTTGGT | 550 | ||
| strA | GCCAAAGGTCGAGGTGTGG CCAGTTCTCTTCGGCGTTAG | 515 | Aminoglycosides resistance | van Overbeek et al. (2002) |
| strB | GACTCCTGCAATCGTCAAGG GCAATGCGTCTAGGATCGAG | 560 | ||
| aadA |
CAGCGCAATGACATTCTTGC GTCGGCAGCGACATCCTTCG |
295 | ||
| gyrA |
CGATGTCGGTCATTGTTG CTTCCGTCAGGTTGTGC |
496 | Fluoroquinolones/Quinolones resistance | MF374502.1 |